A lokacin rani, tsire-tsire suna fuskantar matsi da yawa kamar zafin jiki mai yawa, haske mai ƙarfi, fari (danniya na ruwa), da damuwa na oxidative. Betaine, a matsayin muhimmin mai sarrafa osmotic kuma mai dacewa da solute, yana taka muhimmiyar rawa a jure tsire-tsire ga waɗannan matsalolin lokacin rani. Babban ayyukansa sun haɗa da:

1. Ka'idojin shiga:
Kula da matsa lamba cell turgor:

Yawan zafin jiki da fari suna haifar da shuke-shuke rasa ruwa, haifar da karuwa a cikin cytoplasmic osmotic yuwuwar (zama mai yawa), wanda ke haifar da rashin ruwa a cikin sauƙi da bushewar sel daga kewayen vacuoles ko bangon tantanin halitta tare da ƙarfin ɗaukar ruwa mai ƙarfi. Betaine yana taruwa da yawa a cikin cytoplasm, yadda ya kamata yana rage yuwuwar osmotic na cytoplasm, yana taimaka wa sel su kula da matsananciyar turgor, don haka tsayayya da bushewa da kiyaye amincin tsarin tantanin halitta da aiki.

Daidaitaccen matsi na osmotic vacuolar:

Babban adadin ion inorganic (irin su K ⁺, Cl ⁻, da dai sauransu) suna taruwa a cikin bututu don kula da matsa lamba na osmotic. Betaine yafi wanzuwa a cikin cytoplasm, kuma tarinsa yana taimakawa daidaita bambancin matsa lamba na osmotic tsakanin cytoplasm da vacuoles, yana hana lalacewa ga cytoplasm saboda yawan bushewa.

2. Kare kwayoyin halitta:
Tsayayyen gina jiki:

Babban yanayin zafi na iya haifar da raguwar furotin cikin sauƙi da rashin kunnawa. Kwayoyin Betaine suna ɗaukar caji mai kyau da mara kyau (zwitterionic) kuma suna iya daidaita yanayin halittar sunadaran ta hanyar haɗin hydrogen da hydration, hana ɓarnawa, tarawa, ko raguwa a yanayin zafi mai girma. Wannan yana da mahimmanci don kiyaye ayyukan enzyme, mahimman sunadaran a cikin photosynthesis, da ayyukan sauran sunadaran sunadarai.

Tsarin fim ɗin kariya:

Babban zafin jiki da nau'in iskar oxygen mai amsawa na iya lalata tsarin bilayer na lipid na membranes tantanin halitta (kamar membranes na thylakoid da membranes na plasma), wanda ke haifar da rashin ruwa na membrane mara kyau, yabo, har ma da tarwatsewa. Betaine na iya daidaita tsarin membrane, kula da ruwan sa na yau da kullun da zaɓen zaɓe, da kare mutuncin gabobin photoynthetic da gabobin jiki.

3. Kariyar Antioxidant:
Kula da ma'auni na osmotic kuma rage lalacewar na biyu da damuwa ya haifar.

Tabbatar da tsari da ayyukan enzymes na antioxidant (kamar superoxide dismutase, catalase, ascorbate peroxidase, da dai sauransu), haɓaka ingantaccen tsarin kariya na antioxidant na shuka, kuma a kaikaice yana taimakawa share nau'in oxygen mai amsawa.
Cire nau'in iskar oxygen mai amsawa kai tsaye:

Hasken rana mai ƙarfi da yanayin zafi a lokacin rani na iya haifar da samar da nau'ikan iskar oxygen mai yawa a cikin tsire-tsire, yana haifar da lalacewar iskar oxygen. Ko da yake ita kanta betaine ba ita ce mai ƙarfi antioxidant ba, ana iya samun ta ta hanyar:

4. Kare photosynthesis:
Babban zafin jiki da danniya mai ƙarfi yana haifar da babbar illa ga ainihin tsarin photosynthesis, tsarin photosystem II. Betaine na iya kare membrane na thylakoid, kiyaye kwanciyar hankali na hadaddun photosystem II, tabbatar da aikin sarkar safarar lantarki, da kuma rage hana photosynthesis.

5. A matsayin mai ba da gudummawar methyl:

Betaine yana ɗaya daga cikin mahimman masu ba da gudummawar methyl a cikin rayayyun halittu, waɗanda ke shiga cikin zagayowar methionine. A ƙarƙashin yanayin damuwa, yana iya shiga cikin haɗuwa ko tsarin rayuwa na wasu abubuwa masu amsa damuwa ta hanyar samar da ƙungiyoyin methyl.

A taƙaice, a lokacin bazara mai zafi, babban aikin betaine akan shuke-shuke shine:

Riƙewar ruwa da juriya na fari:magance rashin ruwa ta hanyar tsarin osmotic.
Kariyar juriyar zafi:yana kare sunadaran, enzymes, da membranes tantanin halitta daga lalacewar yanayin zafi.

Juriya ga oxidation:yana haɓaka ƙarfin antioxidant kuma yana rage lalacewar photooxidative.
Kula da photosynthesis:kare gabobin hotuna da kuma kula da samar da makamashi na asali.

Don haka, lokacin da tsire-tsire suka fahimci alamun damuwa kamar yanayin zafi da fari, suna kunna hanyar haɗin betaine (yafi ta hanyar oxidation mataki biyu na choline a cikin chloroplasts), suna tattara betaine na rayayye don haɓaka juriyar damuwa da haɓaka ƙarfin rayuwarsu a cikin matsanancin yanayin bazara. Wasu amfanin gona masu jure fari da gishiri (kamar su sugar gwoza da kansu, alayyahu, alkama, sha'ir, da dai sauransu) suna da ƙarfi wajen tara betaine.

A cikin noman noma, ana kuma amfani da feshin betaine a waje a matsayin biostimulant don haɓaka juriyar amfanin gona (kamar masara, tumatur, chili, da sauransu) zuwa yanayin zafi mai zafi da damuwa na fari.