Domin tantance tasirin ƙara triglyceride a cikin abinci akan samar da furotin na ƙwayoyin cuta na rumen da halayen fermentation na ƙananan ƙananan wutsiya na manya, an gudanar da gwaje-gwaje guda biyu a cikin vitro da kuma a cikin jiki.

Gwajin In vitro: an yi amfani da abincin basal (bisa ga busasshen abu) tare da yawan triglycerides na 0, 2, 4, 6 da 8g / kg a matsayin substrate, sannan aka ƙara ruwan rumen na tumakin manya masu ƙananan tawul, sannan aka saka a cikin zafin 39 ℃ na tsawon awanni 48 a cikin vitro.

Gwajin In vivo: An raba tumaki manya 45 zuwa ƙungiyoyi 5 bazuwar bisa ga nauyinsu na farko (55 ± 5 kg).Glyceryl tributylateAn ƙara na 0, 2, 4, 6 da 8 g/kg (bisa ga busasshen abu) a cikin abincin yau da kullun, kuma an tattara ruwan rumen da fitsari na tsawon kwanaki 18.

Sakamakon Gwaji

1). Tasiri akan ƙimar pH da yawan sinadarin acid mai canzawa

Sakamakon ya nuna cewa ƙimar pH na matsakaicin al'ada ta ragu a layi kuma yawan ƙwayoyin fatty acids masu canzawa (TVFA), acetic acid, butyric acid da branched chain valiable fatty acids (BCVFA) sun ƙaru a layi lokacin datributyl glyceridean ƙara shi a cikin substrate. Sakamakon gwajin in vivo ya nuna cewa shan busasshen abu (DMI) da ƙimar pH sun ragu, kuma yawan TVFA, acetic acid, propionic acid, butyric acid da BCVFA sun ƙaru a layi tare da ƙaratributyl glycerideSakamakon gwajin in vivo ya nuna cewa shan busasshen abu (DMI) da ƙimar pH sun ragu, kuma yawan TVFA, acetic acid, propionic acid, butyric acid da BCVFA sun ƙaru a layi ɗaya tare da ƙara tributyl glyceride.

Sakamakon gwajin in vivo ya nuna cewa shan busasshen abu (DMI) da ƙimar pH sun ragu, kuma yawan TVFA, acetic acid, propionic acid, butyric acid da BCVFA sun ƙaru a layi ɗaya tare da ƙaratributyl glyceride.

2). Inganta yawan lalacewar sinadaran gina jiki

Yawan lalacewar da ake gani a fili na DM, CP, NDF da ADF ya ƙaru a layi ɗaya lokacin datributyl glycerideAn ƙara shi zuwa substrate a cikin vitro.

3). Inganta aikin enzyme mai lalata cellulose

Ƙarintributyrina cikin vitro ya ƙara ayyukan xylanase, carboxymethyl cellulase da microcrystalline cellulase a layi daya. Gwaje-gwajen da aka gudanar a cikin vivo sun nuna cewa triglyceride ya ƙara ayyukan xylanase da carboxymethyl cellulase a layi daya.

4). Inganta samar da furotin na ƙwayoyin cuta

Gwaje-gwajen In vivo sun nuna cewatributyrinyana ƙara yawan allantoin, uric acid a kowace rana da kuma shan purine na ƙwayoyin cuta a cikin fitsari, kuma yana ƙara yawan sinadarin nitrogen na ƙwayoyin cuta na rumen.

Kammalawa

Tributyrinya inganta haɗakar furotin na ƙwayoyin cuta na rumen, yawan sinadarin fatty acid mai canzawa da kuma ayyukan enzymes masu lalata cellulose, sannan ya haɓaka lalacewa da amfani da busassun abu, furotin mai ɗanyen abu, zare mai tsaka tsaki na sabulu da zare mai sabulun acid a cikin abinci.

Yana nuna cewa triglyceride yana da tasiri mai kyau akan yawan furotin na rumen da kuma yadda yake fitowa, kuma yana iya yin tasiri mai kyau akan aikin samar da tumaki manya.