Babban ƙarfin jan hankaliDMPTkumaDMTsababbi ne kuma ingantattun abubuwan jan hankali ga dabbobin ruwa. A cikin wannan binciken, manyan abubuwan jan hankaliDMPTkumaDMTan ƙara su zuwa abincin carp don bincika tasirin abubuwan jan hankali biyu akan ciyarwar carp da haɓaka haɓaka. Sakamakon ya nuna cewa ƙarin abubuwan jan hankali masu ƙarfiDMPTkumaDMTzuwa ciyarwar ta ƙara haɓaka yawan cizon kifin na gwaji kuma yana da tasiri mai mahimmanci na ciyarwa; A lokaci guda, ƙari na nau'i-nau'i daban-daban na manyan abubuwan jan hankaliDMPTkumaDMTzuwa ciyarwar ta ƙara haɓaka ƙimar kiba, ƙayyadaddun ƙimar girma, da adadin tsira na kifin na gwaji, yayin da ƙimar abinci ta ragu sosai. Sakamakon binciken ya kuma nuna cewaDMPTyana da tasiri mai mahimmanci akan jawowa da haɓaka haɓakar irin kifi idan aka kwatanta daDMT.

Mai sha'awar ciyar da dabbar ruwa abu ne da ba na gina jiki ba. Ƙara masu jan hankali don ciyar da kifi zai iya inganta ciyarwar su yadda ya kamata, ƙara yawan abincin su, rage ragowar abinci a cikin ruwa, don haka ya rage gurɓata ruwa a cikin ruwa na kifaye.DMPTkumaDMTabubuwa ne masu aiki a ko'ina a cikin kwayoyin ruwa, suna aiki a matsayin masu ba da gudummawar methyl masu tasiri da mahimmancin matsa lamba na osmotic. Hakanan suna da mahimmancin ciyarwa da haɓaka tasirin haɓaka akan dabbobin ruwa.

Bayan gudanar da binciken da ya dace game da dabbobin ruwa irin su crucian carp, jan snapper, kifin zinare, da kuma tsintsiya, masu binciken Japan sun gano cewa.DMPTkumaDMTsuna da tasiri mai kyau na jan hankali akan ruwa mai kyau da kifin ruwa, crustaceans, da kifin shell. Ƙarfafa ƙarancin ƙima na abubuwan jan hankali masu ƙarfiDMPTkumaDMTa cikin ciyarwa na iya haɓaka ciyarwa da haɓakar kifayen ruwa iri-iri da na ruwa. A cikin wannan gwaji, babban ƙarfin jan hankaliDMPTkumaDMTan ƙara su zuwa abincin carp don nazarin tasirin su akan ciyarwar carp da haɓaka haɓaka, samar da bayanan tunani don yaɗuwar aikace-aikacen waɗannan sabbin abubuwan jan hankali guda biyu a cikin masana'antar abinci da kiwo.

1 Kayayyaki da Hanyoyi

1.1 Kayan gwaji da kifi na gwaji
S. S' - Dimethylacetic acid thiazoleDMT), DMPT
An ɗauko carp ɗin gwajin ne daga gonar kiwo, tare da jikin lafiyayye da ƙayyadaddun bayanai. Kafin a fara gwajin a hukumance, za a yi kiwon kifin na wucin gadi a cikin dakin gwaje-gwaje na tsawon kwanaki 7, inda za a ciyar da su da abincin carp da masana'antar abinci ta samar.
1.2 Abincin gwaji
1.2.1 Abincin Gwajin Lure: Murkushe abincin carp ɗin da masana'antar ciyarwa ta samar, ƙara daidai adadin sitaci na A-sitaci, gauraya daidai gwargwado, kuma a haɗa tare da adadin ruwan da ya dace don yin ƙwallaye masu ƙwanƙwasa 5g kowanne a matsayin abincin ƙungiyar kulawa. A lokaci guda, shirya abincin koto ta fara murkushe abincin carp, ƙara daidai adadin sitaci na alpha, da ƙara koto DMT daDMPTa cikin nau'i biyu na 0.5g/kg da 1g/kg, bi da bi. Mix a ko'ina kuma a haxa tare da daidaitaccen adadin ruwa mai tsafta don yin kowane 5g ball m.
1.2.2 Ciyarwar gwajin girma:

Murkushe abincin carp (daga tushe ɗaya kamar na sama) a cikin foda, wuce shi ta hanyar sieve raga 60, ƙara daidai adadin sitaci alpha, gauraya sosai, haɗuwa da ruwa mai narkewa, matse shi daga sieve cikin granules, iska ta bushe shi don samun abincin ƙungiyar kulawa don gwajin girma. The hadawaDMTda lu'ulu'u na DMPT an narkar da su a cikin ruwa mai narkewa don shirya maganin da ya dace, wanda aka yi amfani da shi don haxa abincin carp mai gauraye da sitaci a cikin granules. Bayan bushewa, an samo abincin ƙungiyar gwaji, tare daDMTda DMPT da aka ƙara a cikin matakan maida hankali guda uku na 0.1g/kg, 0.2g/kg, da 0.3g/kg, bi da bi.

1.3 Hanyar Gwaji
1.3.1 Gwajin Lure: Zaɓi irin kifi na gwaji 5 (tare da matsakaicin nauyin 30g) azaman kifin gwajin. Kafin gwajin, ji yunwa na sa'o'i 24, sa'an nan kuma sanya kifin gwajin a cikin akwatin kifaye na gilashi (tare da girman 40 × 30 × 25cm). Ana gyara abincin lallashi a nesa na 5.0cm daga kasan akwatin kifaye ta amfani da layin da aka dakatar da ke daure da sandar kwance. Kifin ya ciji koto kuma yana girgiza layin, wanda ake watsa shi zuwa mashigin da ke kwance kuma na'urar rikodin dabaran ta rubuta. Ana ƙididdige yawan cizon koto bisa ƙwaryar girgizar kifin 5 da ke cizon koto a cikin mintuna 2. An maimaita gwajin ciyar da kowane rukuni na abinci sau uku, ta yin amfani da sabbin ƙwallan mannewa na ciyarwa kowane lokaci. Ta hanyar gudanar da gwaje-gwaje akai-akai don samun jimlar lamba da matsakaicin mitar baiting, tasirin ciyarwarDMTkuma ana iya kimanta DMPT akan irin kifi.

1.3.2 Gwajin girma yana amfani da aquariums gilashin 8 (girman 55 × 45 × 50cm), tare da zurfin ruwa na 40cm, yanayin ruwa na yanayi, da ci gaba da hauhawar farashi. An ba da kifin gwajin ba da gangan ba kuma an raba su zuwa rukuni biyu don gwajin. Ƙungiya ta farko ta ƙunshi aquariums guda huɗu, ƙididdiga X1 (ƙungiyar sarrafawa), X2 (0.1gDMT/kg feed), X3 (0.2gDMT/kg feed), X4 (0.3gDMT/kg feed); Wani rukuni na 4 aquariums, ƙididdiga Y1 (ƙungiyar sarrafawa), Y2 (0.10g DMPT / kg ciyarwa), Y3 (0.2g DMPT / kg ciyar), Y4 (0.30g DMPT / kg ciyar). Kifi 20 a kowane akwati, ciyar da sau 3 a rana a karfe 8:00, 13:00, da 17:00, tare da adadin ciyarwar yau da kullun na 5-7% na nauyin jiki. Gwajin ya dauki tsawon makonni 6. A farkon da ƙarshen gwajin, an auna jikar kifin gwajin kuma an rubuta adadin rayuwar kowane rukuni.

2.1 Tasirin ciyarwa na DMPT daDMTna carp
Sakamakon ciyarwa na DMPT daDMTa kan carp yana nunawa ta hanyar cizon kifin gwaji a lokacin gwajin na minti 2, kamar yadda aka nuna a cikin Table 1. Gwajin ya gano cewa bayan ƙara DMPT da DMT abinci a cikin akwatin kifaye, kifin gwajin ya nuna sauri ya nuna halin cin abinci mai aiki, yayin da lokacin amfani da abinci na ƙungiyar kulawa, halayen kifin gwajin ya kasance a hankali. Idan aka kwatanta da abincin sarrafawa, kifin na gwaji ya sami ƙaruwa mai yawa a yawan cizon abincin gwajin. DMT da DMPT suna da tasiri mai ban sha'awa akan kifin gwaji.

Matsakaicin ƙimar girma, ƙayyadaddun ƙimar girma, da adadin rayuwa na carp da aka ciyar tare da nau'ikan DMPT daban-daban sun ƙaru sosai idan aka kwatanta da waɗanda aka ciyar da abinci mai sarrafawa, yayin da ƙimar abinci ta ragu sosai. Daga cikin su, ƙari na DMPT zuwa T2, T3, da T4 ya karu da nauyin nauyin yau da kullum na ƙungiyoyi uku da 52.94%, 78.43%, da 113.73%, bi da bi, idan aka kwatanta da ƙungiyar kulawa. Matsakaicin ƙimar T2, T3, da T4 sun ƙaru da 60.44%, 73.85%, da 98.49%, bi da bi, kuma ƙayyadaddun ƙimar girma ya karu da 41.22%, 51.15%, da 60.31%, bi da bi. Adadin rayuwa ya karu daga kashi 90% zuwa 95%, kuma adadin abinci ya ragu da 28.01%, 29.41%, da 33.05%, bi da bi.

3. Kammalawa

A cikin wannan gwaji, koDMTko an ƙara DMPT, mitar ciyarwa, ƙayyadaddun ƙimar girma, da ƙimar yau da kullun na kifin gwaji a cikin kowane rukuni ya karu sosai idan aka kwatanta da ƙungiyar kulawa, yayin da ƙimar abinci ta ragu sosai. Kuma ko DMT ne ko DMPT, haɓakar haɓaka haɓakar haɓaka ya zama mafi mahimmanci tare da haɓaka adadin ƙari a cikin ƙididdiga uku na 0.1g/kg, 0.2g/kg, da 0.3g/kg. A lokaci guda kuma, an kwatanta tasirin ciyarwa da haɓaka haɓakar DMT da DMPT. An gano cewa a karkashin irin wannan maida hankali na aski, yawan ciyarwa, yawan kiba, da takamaiman girma na kifin gwaji a cikin rukunin abinci na DMPT ya karu sosai idan aka kwatanta da rukunin abinci na DMT, yayin da ƙimar abinci ta ragu sosai. Dangantakar magana, DMPT yana da tasiri mai mahimmanci akan jawowa da haɓaka haɓakar irin kifi idan aka kwatanta da DMT. Wannan gwajin ya yi amfani da DMPT da DMT da aka ƙara zuwa abincin carp don bincika ciyarwarsu da haɓakar tasirin su. Sakamako ya nuna cewa DMPT da DMT suna da fa'idar aikace-aikace a matsayin sabon ƙarni na abubuwan jan hankali na dabbobin ruwa.