Masu jan hankali masu ƙarfi sosaiDMPTkumaDMTsabbin abubuwa ne masu jan hankali ga dabbobin ruwa. A cikin wannan binciken, abubuwa masu jan hankali masu ƙarfiDMPTkumaDMTan ƙara su a cikin abincin kifi don bincika tasirin masu jan hankali guda biyu akan ciyar da kifi da haɓaka girma. Sakamakon ya nuna cewa ƙarin masu jan hankali masu ƙarfi.DMPTkumaDMTga abincin ya ƙara yawan cizon kifin gwaji sosai kuma yana da tasirin ciyarwa mai mahimmanci; A lokaci guda, ƙarin yawan abubuwan jan hankali masu ƙarfi daban-daban ya ƙaru sosai.DMPTkumaDMTGa abincin ya ƙara yawan nauyin da ke cikinsa, takamaiman girman girma, da kuma adadin tsira na kifin gwaji, yayin da adadin abincin ya ragu sosai. Sakamakon binciken ya kuma nuna cewaDMPTyana da tasiri mai mahimmanci wajen jawo hankali da haɓaka ci gaban kifi idan aka kwatanta daDMT.

Maganin jan hankalin dabbobi a cikin ruwa wani ƙari ne da ba shi da gina jiki. Ƙara abubuwan jan hankali ga ciyar da kifi zai iya haɓaka ciyarwarsu yadda ya kamata, ƙara yawan abincin da suke ci, rage abincin da ya rage a cikin ruwa, don haka rage gurɓataccen yanayi a cikin ruwan kiwon kamun kifi.DMPTkumaDMTsinadarai ne masu aiki da yawa da ke cikin halittun ruwa, suna aiki a matsayin masu ba da gudummawar methyl masu tasiri da kuma masu daidaita matsin lamba na osmotic. Hakanan suna da tasiri mai mahimmanci na ciyarwa da haɓaka girma ga dabbobin ruwa.

Bayan gudanar da bincike mai dacewa kan dabbobin ruwa kamar crucian carp, red snapper, goldfish, da spotted shrimp, masu binciken Japan sun gano cewaDMPTkumaDMTsuna da kyakkyawan tasirin jan hankali ga kifayen ruwa da na ruwa, crustaceans, da kuma kifin shellfish. Ƙara ƙarancin yawan abubuwan jan hankali masu ƙarfi.DMPTkumaDMTa cikin abincin da ake ci zai iya hanzarta ciyarwa da girma nau'ikan kifaye daban-daban na ruwa da na ruwa. A cikin wannan gwajin, abubuwan jan hankali masu ƙarfi masu ƙarfiDMPTkumaDMTan ƙara su a cikin abincin kifi don nazarin tasirinsu akan ciyar da kifi da haɓaka girma, suna ba da bayanai na asali don amfani da waɗannan sabbin masu jan hankali guda biyu a masana'antar ciyar da kifi.

1 Kayan aiki da Hanyoyi

1.1 Kayan gwaji da kifi na gwaji
S. S' - Dimethylacetic acid thiazole (DMT), DMPT
An ɗauko kifin gwajin kifi daga gonar kiwon kamun kifi, mai lafiyayyen jiki da kuma cikakkun bayanai. Kafin a fara gwajin a hukumance, za a yi kifin gwajin na ɗan lokaci a dakin gwaje-gwaje na tsawon kwanaki 7, inda za a ciyar da su da abincin kifi da masana'antar ciyarwa ta samar.
1.2 Ciyarwar gwaji
1.2.1 Abincin gwajin jan hankali: A niƙa abincin kifi da masana'antar ciyarwa ta samar, a ƙara daidai adadin sitaci na A, a gauraya daidai gwargwado, sannan a gauraya da ruwa mai kyau don yin ƙwallo mai manne 5g kowanne yayin da ƙungiyar sarrafawa ke ciyarwa. A lokaci guda, a shirya abincin kifi ta hanyar da farko a niƙa abincin kifi, a ƙara daidai adadin sitaci na alpha, sannan a ƙara abincin kifi DMT daDMPTA cikin ruwa mai yawa guda biyu na 0.5g/kg da 1g/kg, bi da bi. A gauraya daidai gwargwado sannan a gauraya da ruwa mai narkewa daidai gwargwado don yin kowanne ƙwallo mai mannewa na 5g.
1.2.2 Ciyar da gwajin girma:

A niƙa abincin kifi (daga tushen iri ɗaya kamar yadda yake a sama) a cikin foda, a ratsa shi ta cikin sieve mai kauri 60, a ƙara daidai adadin sitaci na alpha, a gauraya sosai, a gauraya da ruwan da aka tace, a matse shi daga sieve zuwa granules, sannan a busar da shi a iska don samun abincin ƙungiyar sarrafawa don gwajin girma.DMTkuma an narkar da lu'ulu'u na DMPT a cikin ruwan da aka tace don shirya ruwan da ya dace, wanda aka yi amfani da shi don haɗa abincin kifi da sitaci da aka haɗa sosai cikin granules. Bayan bushewa, an sami abincin rukuni na gwaji, tare daDMTkuma an ƙara DMPT a cikin matakai uku na tattarawa na 0.1g/kg, 0.2g/kg, da 0.3g/kg, bi da bi.

1.3 Hanyar Gwaji
1.3.1 Gwajin Lalata: Zaɓi kifin gwaji guda 5 (tare da matsakaicin nauyin 30g) a matsayin kifin gwaji. Kafin gwajin, a yi yunwa na tsawon awanni 24, sannan a sanya kifin gwaji a cikin akwatin kifaye na gilashi (mai girman 40 × 30 × 25cm). Ana gyara abincin jan hankali a nisan 5.0cm daga ƙasan akwatin kifaye ta amfani da layin da aka daure a kan sandar kwance. Kifin yana cizon koto kuma yana girgiza layin, wanda aka aika zuwa sandar kwance kuma mai rikodin ƙafa yana rikodin shi. Ana ƙididdige yawan cizon koto bisa ga girgizar kifin gwaji guda 5 da ke cizon koto cikin mintuna 2. An maimaita gwajin ciyarwa ga kowane rukuni na abinci sau uku, ta amfani da ƙwallan manne da aka shirya a kowane lokaci. Ta hanyar yin gwaje-gwaje akai-akai don samun jimlar adadi da matsakaicin mitar koto, tasirin ciyarwa naDMTkuma ana iya kimanta DMPT akan carp.

1.3.2 Gwajin girma yana amfani da gilasan kifin aquarium guda 8 (girman 55 × 45 × 50cm), tare da zurfin ruwa na 40cm, zafin ruwan halitta, da kuma ci gaba da hauhawar farashin kaya. An rarraba kifin gwaji bazuwar kuma an raba shi zuwa ƙungiyoyi biyu don gwajin. Rukunin farko ya ƙunshi kifin aquarium guda huɗu, waɗanda aka yiwa lamba X1 (ƙungiyar sarrafawa), X2 (0.1gDMT/kg ciyarwa), X3 (0.2gDMT/kg ciyarwa), X4 (0.3gDMT ciyarwa/kg); Wani rukuni na kifin aquarium guda 4, waɗanda aka yiwa lamba Y1 (ƙungiyar sarrafawa), Y2 (0.10g ciyarwa DMPT/kg), Y3 (0.2g ciyarwa DMPT/kg ciyarwa), Y4 (0.30g ciyarwa DMPT/kg ciyarwa). Kifi 20 a kowace akwati, ana ciyar da su sau 3 a rana a 8:00, 13:00, da 17:00, tare da yawan ciyarwa na yau da kullun na 5-7% na nauyin jiki. Gwajin ya ɗauki tsawon makonni 6. A farkon gwajin da ƙarshensa, an auna nauyin jikewar kifin gwajin kuma an rubuta adadin tsirar kowane rukuni.

2.1 Tasirin ciyarwa na DMPT daDMTa kan kifi
Tasirin ciyarwa na DMPT daDMTAna nuna yawan cizon kifi a kan kifi mai kama da ...

Yawan karuwar nauyi, takamaiman girman girma, da kuma yawan tsira na kifin carp da aka ciyar da shi tare da yawan DMPT daban-daban sun karu sosai idan aka kwatanta da waɗanda aka ciyar da su tare da abincin sarrafawa, yayin da aka rage yawan abincin sosai. Daga cikinsu, ƙarin DMPT zuwa T2, T3, da T4 ya ƙara yawan nauyin yau da kullun na ƙungiyoyi uku da kashi 52.94%, 78.43%, da 113.73%, bi da bi, idan aka kwatanta da rukunin sarrafawa. Yawan karuwar nauyi na T2, T3, da T4 ya karu da kashi 60.44%, 73.85%, da 98.49%, bi da bi, kuma takamaiman ƙimar girma ya karu da kashi 41.22%, 51.15%, da 60.31%, bi da bi. Yawan tsira ya karu daga kashi 90% zuwa 95%, kuma adadin ciyarwar ya ragu da kashi 28.01%, 29.41%, da 33.05%, bi da bi.

3. Kammalawa

A cikin wannan gwaji, koDMTko kuma an ƙara DMPT, yawan ciyarwa, takamaiman yawan girma, da kuma yawan nauyin da ake samu a kowace rana na kifin gwaji a kowace ƙungiya ya ƙaru sosai idan aka kwatanta da ƙungiyar kulawa, yayin da adadin ciyarwa ya ragu sosai. Kuma ko DMT ne ko DMPT, tasirin haɓaka girma ya zama mafi mahimmanci tare da ƙaruwar adadin ƙarin a cikin yawan uku na 0.1g/kg, 0.2g/kg, da 0.3g/kg. A lokaci guda, an yi kwatanta tasirin ciyarwa da haɓaka girma na DMT da DMPT. An gano cewa a ƙarƙashin yawan aski iri ɗaya, yawan ciyarwa, ƙimar ƙaruwar nauyi, da takamaiman ƙimar girma na kifin gwaji a cikin ƙungiyar ciyarwa ta DMPT sun ƙaru sosai idan aka kwatanta da ƙungiyar ciyarwa ta DMT, yayin da adadin ciyarwa ya ragu sosai. A takaice dai, DMPT yana da tasiri mai mahimmanci akan jawo da haɓaka haɓakar kifin kifi idan aka kwatanta da DMT. Wannan gwajin ya yi amfani da DMPT da DMT da aka ƙara a cikin abincin kifi don bincika tasirin ciyarwa da haɓaka girma. Sakamakon ya nuna cewa DMPT da DMT suna da fa'idodi masu yawa na amfani a matsayin sabon ƙarni na masu jan hankalin dabbobi na ruwa.